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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
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INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 7 Documents
Search results for , issue "Vol 5 No 2 (2000): June 2000" : 7 Documents clear
ANALISIS STATISTIK PENGUJI KECENDERUNGAN POPULASI DAN VARIASI TIPE HABITAT BUAYA Crocodylusporosus DI DAERAH PASANG SURUT TELUK ARGUNI IRIAN JAYA Hellen Kurniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/465

Abstract

The population ecology of saltwater (Crocodylusporosus) in Arguni Bay, Irian Jaya was studied during 19990-1998 period. In this study, the habitas were divided into five types (1. Dominant vegetation Nypafructicansand primary forest; 2.Dominant vegetations N. fructicans; 4.Mangrove; 5. Lake with dominate vegetation N. fructicans and mangrove, along the lake bank was covered by Barringtonia spp, Ficusspp and Callophylum spp, whereas almost at lake offshore was covered by lotus floating plant). Result of GLM statistical analysis showed there were no significant (p>0.05) interaction between habitat type and survey time, neither on the habitat type and time of survey. The Duncan test on habitat showed a grouping in three categories (A, AB, and B) in which habitat 5 was at first ranking. However, there was no grouping on time of survey. From this study it was concluded that mangrove habitat was essential for C.porosus life and used as a nursery ground.
AKTIVITAS KONTRASEPSI DAN INTERSEPSI EKSTRAK METANOL DAUN BENALU Dendrophthoe petandra L PADA MENCIT G N Astika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/467

Abstract

It had been reported that leaf of Dendrophthoe petandra contained a flavonol glycoside of which quercetion was its aglycone. In-vitro study in the activity of quercetin indicated that quercetin inhibited the activity of sperm hyaluronidase. So it meant quercetin inhibited sperm penetration into cumulus. Other study proved that quercetin inhibited the activity of cytochrome P-450 III A 4 enzyme which catalyzed the reversible hydroxylation of estradiol-17β into estrone and futher into estriol. The elevation of estradiol serum level disturbed gamete and zygote transport in oviduct causing premature expulsion of zygote into uterus. This research was designed to provide information about the anti-fertility activity of methanolic extract of leaf of Dendrophthoe petandra, focused on its contraceptive and interceptive activity. Virgin female Balc-C mice were used as the test animals. Each study had two group of mice which were treated with 150 mg/30 g and 200 mg/30 g extract respectively. Control group were treated with respective vehicle at 1 ml/30 g. statistical analysis of data (p<0.05) concluded that the extract had contraceptive and interceptive activity. According to the criterion given by Lee andChi (1985), this methanolic extract had a priority for further study.
PENGHILANGAN GEN Lacz DARI PLASMID pUKC 815 UNTUK MEMBENTUK VEKTOR EKSPRESI DI RAGI Saccharomyces cereviceae Ahmad Thontowi; Ni Nyoman T P; Mariatun Loegito
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/469

Abstract

The pUKC 815 plasmid was a shuttle vector. This plasmid had the genetic marker to the urasil (URA3) in yeast and ampicillin resistanced marked in E. coli. The pUKC 815 plasmid also had a promotor gene PGK. The PGK promotor was expected to inducting the expression of amylase gene. Expression of amylase gene in S. cereviceaecould be do by preparing the pUKC 815 plasmid as an expression vector through reducing the LacZ gene then it would be replace by the amylase gene. This research used several methods. These method were isolate the pUKC 815 plasmid, the digestion of the pUKC 815 DNAwith the BamHI restriction enzyme, electrolution, ligation and the transformation of 8.000 pb fragment pUKC 815 plasmid in to the cell of E. coli DH5a. the result shown that the expression vector in yeast (S. cereviceae) from the pUKC 815 plasmid with the 8.000 pb was formed.
AKTIVITAS ANTIBAKTERI NBENZOILAMOKSISILIN DAN N34DIKLOROBENZOILAMOKSISILIN TERHADAP Staphylococcus ATCC 25923 DAN Escherichia coli ATCC 35218 Harry Santosa; Bambang Soekardjo; Ami Soewandi J S
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/470

Abstract

N-Benzolamoxicillin and N-3,4-dichlorobenzolamoxicillin were synthesized from amoxicillin by acylation with benzoylchloride and 3,4-dichlorobenzoylchloride respectively. Structure characterization of the amoxicillin derivative compounds were done by UV, IR and 1H-NMR spectroscopy. Both derivative compound has antibacterial antivity against Staphylococcus aureus and Escherichia coli. The activity of N-3,4-dichlorobenzolamoxicillin is more active than N-Benzolamoxicillin.
CHARACTERIZATION OF ENVELOPE E PROTEIN OF DENGUE VIRUS INDNESIA ISOLATE Rantam F A; Soetjipto Soetjipto; Dachlan Y P
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/472

Abstract

Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein characterization from dengue virus infected C5/36 cells for vaccine candidate. We found that E protein induced antibody response. The data of the reactivity on the nitrocellulose membrane between E protein against polyclonal and monoclonal antibodies on western blot analysis, shown that the E protein whisc was once of dengue virus protein has immunogenic. The Indonesia isolate had no difference with the Phillipine isolate. The result suggested that all of the E protein dengue virus strain have peptides overlapping. Also promising for he production of immunogenic particulate proteins, especially DEN protein for the four strains.
INFLUENCE OF ISONIAZID ON THE METABOLISM OF RIFAMPICIN ON MT HEPATOCYTES Fasich Fasich; Suharjono Suharjono; I Ketut Mandikin; Mulja Hadi Santosa
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/474

Abstract

Research on the influence of isoniazid on rifampicin metabolism using rat hepatocytes has been done. As the system metabolism, hepatocytes suspension was prepared by perfusion in the calcium binding media containing EDTA, citrate and glycine was used. In the experiment, combination of rifampicin and INH on various doses was incubated in those metabolism system at 30oC from 0 and up to 180 minutes duration. Then, analysis was done by thin layer chromathography and densitomeric method for each incubation intervals. Result calculated on the base of variation of rifampicin AUC-value beginning from 0 up to 180 minutes showed that isoniazid with concentration 5 µl, 10 µl, 15 µl, and 20 µl did not influence the metabolism of rifampicin (10 µl/ml) as showed by no significance changes of AUC value of rifampicin for α = 0.05.
POLA PENIJAHAN UDANG AIR TAWAR Macrobrachium sintangense De Man: PENGAMATAN SEMUSIM DI RANU KLINDUNGAN PASURUAN Bambang Irawan; Indinah Indinah; Hani Sudarmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/475

Abstract

The spawning pattern of Macrobrachium sintangense (de man) population in Klindungan pond (Ranu Klindungan) was observed. The number of eggs carrying female was count and high proportion of these female was found in August and February. This result indicate that there was two period of spawning in this population.

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